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polyclonal rabbit anti phospho stat3 s727 cell signaling technology  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal rabbit anti phospho stat3 s727 cell signaling technology
    Polyclonal Rabbit Anti Phospho Stat3 S727 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti%E2%80%93stat2/Stat2+Rabbit+mAb/pmc11949035__jciinsight___10___187825___s136-42-70-73
    Average 96 stars, based on 248 article reviews
    polyclonal rabbit anti phospho stat3 s727 cell signaling technology - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Fostamatinib (R788), a spleen tyrosine kinase inhibitor, sensitizes pancreatic cancer cells to oncolytic vesicular stomatitis virus
    Article Snippet: .. Membranes were then incubated in TBS-T with 5% BSA or milk with 0.02% sodium azide and a 1:5,000 dilution of rabbit polyclonal anti-VSV antibodies (raised against VSV virions), a 1:1,000 dilution of rabbit anti-phospho-STAT1 (catalog number 9167S, Cell Signaling), a 1:1,000 dilution of rabbit anti-STAT1 (catalog number 14994T, clone D1K9Y, Cell Signaling), a 1:1,000 dilution of rabbit anti-phospho-STAT2 (catalog number PA5-97361clone p-Y690, Invitrogen), a 1:1,000 dilution of rabbit anti-STAT2 (catalog number 4594, Cell Signaling), a 1:1,000 dilution of rabbit anti-phospho-STAT3 (catalog number 9134P, clone Y705, Cell Signaling), a 1:1,000 dilution of mouse anti-STAT3 (catalog number 9139P, clone 124H6, Cell Signaling), rabbit anti-phospho-MAPK (catalog number 4370S,clone p-T202/Y204, Cell Signaling), a 1:1,000 dilution of rabbit anti-MAPK (catalog number 9102L Cell Signaling), rabbit anti-phospho-SYK (catalog number 2710Sclone p-Y525, Cell Signaling), a 1:1,000 dilution of rabbit anti-SYK (catalog number 13198S, Cell Signaling), a 1:1,000 dilution of rabbit anti-OAS2 (catalog number PA5-65422 Cell Signaling), a 1:1,000 dilution of rabbit anti-GAPDH (catalog number 1E6D9 Proteintech), 1:1,000 dilution of rabbit anti-phsopho-TBK1/NAK (catalog number 5483P, clone S172, Cell Signaling), and a 1:1,000 dilution of rabbit anti-MX1 (catalog number 13750-1-AP, Proteintech). .. Starbright Blue 700 goat anti-rabbit (Bio-Rad, 12004161) immunoglobulin G (IgG) fluorescent secondary antibodies at 1:5,000 dilutions were used for fluorescent western blotting detection using the Chemidoc MP imaging system from Bio-Rad.

    Article Title: Distinct ZIKV strain signatures and type I IFN modulation reveal a protective role of brain endothelial interferon signaling in vitro and in vivo
    Article Snippet: The samples were subjected to a 10% polyacrylamide gel electrophoresis (SDS-PAGE), followed by transfer to a nitrocellulose membrane (Merck). .. The membranes were blocked with 10% TBS containing 5% bovine serum albumin (BSA; Merck) for 1 h, and incubated with the antibodies anti-p-STAT1, anti-STAT1, anti-p-STAT2, anti-STAT2, anti-RIG-I, anti-TLR-3, and anti-β-actin (Cell Signaling Technology, Danvers, MA), overnight at 4 0 C. The membranes were washed and incubated with the respective HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories Inc., West Grove, PA) for 1 h and developed using the ECLTM Prime Western Blotting System kit (GE Healthcare, Boston, MA). .. The ratio between phosphorylated and nonphosphorylated proteins, as well as the ratio of total protein expression (sum of phosphorylated and nonphosphorylated) and β-actin, were calculated using ImageJ software.

    Article Title: A 20-amino-acid Cardiovirus protein exhibits cytokine-mimicry activity to regulate viral replication.
    Article Snippet: The PVDF membrane was pre-soaked in methanol (Nacalai Tesque Inc., 21915-93), and transferred using transfer buffer (25mM Tris-base, 192mM glycine (Nacalai Tesque Inc., 17109-35), 20% methanol). .. After the transfer process, the PVDF membrane was blocked using 5% BSA (Nacalai Tesque Inc., 01863-48) in 1X TBS-T (20mM Tris-HCl pH 8.0, 150mM NaCl, 0.1% Tween 20 (Nacalai Tesque Inc., 35624-15) for 60 min, and incubated with primary antibodies (diluted with 5% BSA in TBS-T) overnight at 4 ◦ C. Dilution of primary antibodies: Anti-GAPDH (Santa Cruz Biotechnology, sc-32233): 1:1000, Anti-caspase-8 (CST, #9746): 1:500, Anti-Cleaved PARP (CST, #9541): 1:1000, Anti-VPg EMCV (IBL, This study): 1:5000, Anti-Mengo 3DPol (Santa Cruz Biotechnology, sc-65633): 1:500, Anti-iNOS (Santa Cruz Biotechnology, sc-7271): 1:500, Anti-STAT1 (CST, #9172): 1:1000, Anti-pSTAT1 (Y701) (CST, #9167): 1:1000, Anti-pSTAT2 (Y690) (CST, #4441): 1:1000, Anti-Myc-Tag (CST, #2276): 1:1000, Anti-IκBα (Santa Cruz Biotechnology, sc-371): 1:500, Anti-HSP70 (Santa Cruz Biotechnology, sc-32239): 1:1000, Anti-IFNGR1 (Proteintech, 10808-1-AP): 1:1000, Anti-IFNGR2 (Proteintech, 10266-1-AP): 1:500. .. The PVDF membrane was washed 3 times for 10 min using TBS-T, then incubated for 1 h at room temperature using AP-conjugated secondary antibodies, either anti-mouse (CST, #7056) or anti-rabbit (CST, #7054) diluted using 5% BSA (1:4000 concentration) in TBS-T. After washing 3 times for 10 min using TBS-T, the membrane was washed quickly using TBS, bands were detected using BCIP-NBT solution (Nacalai Tesque Inc., 19880-84), and the reaction was stopped using purified water.

    Western Blot:

    Article Title: Distinct ZIKV strain signatures and type I IFN modulation reveal a protective role of brain endothelial interferon signaling in vitro and in vivo
    Article Snippet: The samples were subjected to a 10% polyacrylamide gel electrophoresis (SDS-PAGE), followed by transfer to a nitrocellulose membrane (Merck). .. The membranes were blocked with 10% TBS containing 5% bovine serum albumin (BSA; Merck) for 1 h, and incubated with the antibodies anti-p-STAT1, anti-STAT1, anti-p-STAT2, anti-STAT2, anti-RIG-I, anti-TLR-3, and anti-β-actin (Cell Signaling Technology, Danvers, MA), overnight at 4 0 C. The membranes were washed and incubated with the respective HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories Inc., West Grove, PA) for 1 h and developed using the ECLTM Prime Western Blotting System kit (GE Healthcare, Boston, MA). .. The ratio between phosphorylated and nonphosphorylated proteins, as well as the ratio of total protein expression (sum of phosphorylated and nonphosphorylated) and β-actin, were calculated using ImageJ software.

    Article Title: Crimean-Congo hemorrhagic fever virus NSm protein inhibits the type I interferon signaling by binding to STAT2.
    Article Snippet: .. Western blotting was performed as previously described using the following antibodies [10,12,13]: anti-HA (clone 3F10; Roche), anti-His (clone 9F2; Fujifilm Wako), anti-FLAG (catalog no. PM020; MBL), anti-VSIV M (clone 23H12; Kerafast), anti-VSIV G (clone P5D4; Sigma-Aldrich), anti-STAT2 (catalog no.: D9J7L; Cell Signaling Technology), anti-STAT1 (catalog no.: D19KY; Cell Signaling Technology), anti-STAT2 (phosphor Y690) (catalog no.: D3P2P; Cell Signaling Technology), anti-STAT1 (phosphor Y701) (catalog no.: D4A7; Cell Signaling Technology), anti-CCHFV Gn (in-house production) or anti-β-actin (catalog no. AC-15; Sigma-Aldrich). .. The band intensities of VSIV-G, VSIV-M, STAT1, pSTAT1, STAT2, pSTAT2, NSm-FLAG, and β-actin were quantified using ImageJ software (National Institutes of Health).

    Article Title: Crimean-Congo hemorrhagic fever virus NSm protein inhibits the type I interferon signaling by binding to STAT2
    Article Snippet: .. Western blotting was performed as previously described using the following antibodies [ , , ]: anti-HA (clone 3F10; Roche), anti-His (clone 9F2; Fujifilm Wako), anti-FLAG (catalog no. PM020; MBL), anti-VSIV M (clone 23H12; Kerafast), anti-VSIV G (clone P5D4; Sigma-Aldrich), anti-STAT2 (catalog no.: D9J7L; Cell Signaling Technology), anti-STAT1 (catalog no.: D19KY; Cell Signaling Technology), anti-STAT2 (phosphor Y690) (catalog no.: D3P2P; Cell Signaling Technology), anti-STAT1 (phosphor Y701) (catalog no.: D4A7; Cell Signaling Technology), anti-CCHFV Gn (in-house production) or anti-β-actin (catalog no. AC-15; Sigma-Aldrich). .. The band intensities of VSIV-G, VSIV-M, STAT1, pSTAT1, STAT2, pSTAT2, NSm-FLAG, and β-actin were quantified using ImageJ software (National Institutes of Health).

    Membrane:

    Article Title: A 20-amino-acid Cardiovirus protein exhibits cytokine-mimicry activity to regulate viral replication.
    Article Snippet: The PVDF membrane was pre-soaked in methanol (Nacalai Tesque Inc., 21915-93), and transferred using transfer buffer (25mM Tris-base, 192mM glycine (Nacalai Tesque Inc., 17109-35), 20% methanol). .. After the transfer process, the PVDF membrane was blocked using 5% BSA (Nacalai Tesque Inc., 01863-48) in 1X TBS-T (20mM Tris-HCl pH 8.0, 150mM NaCl, 0.1% Tween 20 (Nacalai Tesque Inc., 35624-15) for 60 min, and incubated with primary antibodies (diluted with 5% BSA in TBS-T) overnight at 4 ◦ C. Dilution of primary antibodies: Anti-GAPDH (Santa Cruz Biotechnology, sc-32233): 1:1000, Anti-caspase-8 (CST, #9746): 1:500, Anti-Cleaved PARP (CST, #9541): 1:1000, Anti-VPg EMCV (IBL, This study): 1:5000, Anti-Mengo 3DPol (Santa Cruz Biotechnology, sc-65633): 1:500, Anti-iNOS (Santa Cruz Biotechnology, sc-7271): 1:500, Anti-STAT1 (CST, #9172): 1:1000, Anti-pSTAT1 (Y701) (CST, #9167): 1:1000, Anti-pSTAT2 (Y690) (CST, #4441): 1:1000, Anti-Myc-Tag (CST, #2276): 1:1000, Anti-IκBα (Santa Cruz Biotechnology, sc-371): 1:500, Anti-HSP70 (Santa Cruz Biotechnology, sc-32239): 1:1000, Anti-IFNGR1 (Proteintech, 10808-1-AP): 1:1000, Anti-IFNGR2 (Proteintech, 10266-1-AP): 1:500. .. The PVDF membrane was washed 3 times for 10 min using TBS-T, then incubated for 1 h at room temperature using AP-conjugated secondary antibodies, either anti-mouse (CST, #7056) or anti-rabbit (CST, #7054) diluted using 5% BSA (1:4000 concentration) in TBS-T. After washing 3 times for 10 min using TBS-T, the membrane was washed quickly using TBS, bands were detected using BCIP-NBT solution (Nacalai Tesque Inc., 19880-84), and the reaction was stopped using purified water.



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    Image Search Results


    Journal: iScience

    Article Title: Distinct pathways utilized by METTL3 to regulate antiviral innate immune response

    doi: 10.1016/j.isci.2024.111071

    Figure Lengend Snippet:

    Article Snippet: Rabbit polyclonal anti-p-STAT2 , Cell Signaling Technology , Cat# 4441; RRID: AB_2198445.

    Techniques: Virus, Recombinant, Transfection, Reverse Transcription, Enzyme-linked Immunosorbent Assay, Luciferase, SYBR Green Assay, Isolation, shRNA, Plasmid Preparation, Software

    Primers sequence used in SYBR-based reverse transcription-quantitative PCR.

    Journal: Oncology Letters

    Article Title: Machine learning model reveals roles of interferon‑stimulated genes in sorafenib‑resistant liver cancer

    doi: 10.3892/ol.2024.14571

    Figure Lengend Snippet: Primers sequence used in SYBR-based reverse transcription-quantitative PCR.

    Article Snippet: After blocking the membrane in TBS containing 5% skim milk for 1 h. The antibodies used for immunoblotting were as follows: rabbit monoclonal anti-STAT1 (Cell signaling Technology, Cat#9176S), rabbit monoclonal anti-PY STAT1 (Cell signaling Technology, Cat#9167S), rabbit polyclonal anti-STAT2 (Cell signaling Technology, Cat#4594S), rabbit polyclonal anti-PY STAT2 (Cell signaling Technology, Cat#4441S), rabbit monoclonal IRF9 (Cell signaling Technology, Cat#28492), and horseradish peroxidase-conjugated secondary antibody (1:5,000).

    Techniques: Sequencing

    Increased IRF9 expression in sorafenib-resistant liver cancer cells. (A) Procedure for establishing sorafenib-resistant liver cancer cells. (B) liver cancer cells were treated with an increasing dose of sorafenib for 24 h. Cell viability was measured by MTT assay. (C) Protein levels of STAT1, STAT2 and IRF9 from immunoblotting. (D) mRNA levels of STAT1, STAT2 and IRF9 from reverse transcription-quantitative PCR. *P<0.05 and **P<0.01 vs. liver cancer cell lines (Huh-7 and HepG2). IRF9, interferon regulatory factor 9; wks, weeks; p-, phosphorylated.

    Journal: Oncology Letters

    Article Title: Machine learning model reveals roles of interferon‑stimulated genes in sorafenib‑resistant liver cancer

    doi: 10.3892/ol.2024.14571

    Figure Lengend Snippet: Increased IRF9 expression in sorafenib-resistant liver cancer cells. (A) Procedure for establishing sorafenib-resistant liver cancer cells. (B) liver cancer cells were treated with an increasing dose of sorafenib for 24 h. Cell viability was measured by MTT assay. (C) Protein levels of STAT1, STAT2 and IRF9 from immunoblotting. (D) mRNA levels of STAT1, STAT2 and IRF9 from reverse transcription-quantitative PCR. *P<0.05 and **P<0.01 vs. liver cancer cell lines (Huh-7 and HepG2). IRF9, interferon regulatory factor 9; wks, weeks; p-, phosphorylated.

    Article Snippet: After blocking the membrane in TBS containing 5% skim milk for 1 h. The antibodies used for immunoblotting were as follows: rabbit monoclonal anti-STAT1 (Cell signaling Technology, Cat#9176S), rabbit monoclonal anti-PY STAT1 (Cell signaling Technology, Cat#9167S), rabbit polyclonal anti-STAT2 (Cell signaling Technology, Cat#4594S), rabbit polyclonal anti-PY STAT2 (Cell signaling Technology, Cat#4441S), rabbit monoclonal IRF9 (Cell signaling Technology, Cat#28492), and horseradish peroxidase-conjugated secondary antibody (1:5,000).

    Techniques: Expressing, MTT Assay, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction

    U-ISGs unresponsiveness depends on STAT1, STAT2 and IRF9 in Huh-7-SR cells. (A) Huh-7-SR cells were transfected with si-control, si-STAT1, si-STAT2, and si-IRF9. Then, 48 h after transfection, cells were harvested and immunoblotting of STAT1, STAT2 and IRF9 was performed. (B) mRNA levels of U-ISGs were measured by reverse transcription-quantitative PCR. (C) After transfection, Huh-7-SR cells were treated with an increasing dose of sorafenib for 24 h. **P<0.01 vs. siControl. IRF, interferon regulatory factor; si, small interfering; OAS1; oligoadenylate synthetase 1; IFI27, Interferon Alpha Inducible Protein 27.

    Journal: Oncology Letters

    Article Title: Machine learning model reveals roles of interferon‑stimulated genes in sorafenib‑resistant liver cancer

    doi: 10.3892/ol.2024.14571

    Figure Lengend Snippet: U-ISGs unresponsiveness depends on STAT1, STAT2 and IRF9 in Huh-7-SR cells. (A) Huh-7-SR cells were transfected with si-control, si-STAT1, si-STAT2, and si-IRF9. Then, 48 h after transfection, cells were harvested and immunoblotting of STAT1, STAT2 and IRF9 was performed. (B) mRNA levels of U-ISGs were measured by reverse transcription-quantitative PCR. (C) After transfection, Huh-7-SR cells were treated with an increasing dose of sorafenib for 24 h. **P<0.01 vs. siControl. IRF, interferon regulatory factor; si, small interfering; OAS1; oligoadenylate synthetase 1; IFI27, Interferon Alpha Inducible Protein 27.

    Article Snippet: After blocking the membrane in TBS containing 5% skim milk for 1 h. The antibodies used for immunoblotting were as follows: rabbit monoclonal anti-STAT1 (Cell signaling Technology, Cat#9176S), rabbit monoclonal anti-PY STAT1 (Cell signaling Technology, Cat#9167S), rabbit polyclonal anti-STAT2 (Cell signaling Technology, Cat#4594S), rabbit polyclonal anti-PY STAT2 (Cell signaling Technology, Cat#4441S), rabbit monoclonal IRF9 (Cell signaling Technology, Cat#28492), and horseradish peroxidase-conjugated secondary antibody (1:5,000).

    Techniques: Transfection, Control, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction

    Mechanisms of U-ISGF3 complex in sorafenib resistance. U-ISGF complex, unphosphorylated interferon-stimulated gene factor-3; U-STAT1, unphosphorylated signal transducer and activator of transcription 1; U-STAT2, unphosphorylated signal transducer and activator of transcription 2; IRF9, interferon regulatory factor 9; U-ISGs, Unphosphorylated interferon-stimulated genes.

    Journal: Oncology Letters

    Article Title: Machine learning model reveals roles of interferon‑stimulated genes in sorafenib‑resistant liver cancer

    doi: 10.3892/ol.2024.14571

    Figure Lengend Snippet: Mechanisms of U-ISGF3 complex in sorafenib resistance. U-ISGF complex, unphosphorylated interferon-stimulated gene factor-3; U-STAT1, unphosphorylated signal transducer and activator of transcription 1; U-STAT2, unphosphorylated signal transducer and activator of transcription 2; IRF9, interferon regulatory factor 9; U-ISGs, Unphosphorylated interferon-stimulated genes.

    Article Snippet: After blocking the membrane in TBS containing 5% skim milk for 1 h. The antibodies used for immunoblotting were as follows: rabbit monoclonal anti-STAT1 (Cell signaling Technology, Cat#9176S), rabbit monoclonal anti-PY STAT1 (Cell signaling Technology, Cat#9167S), rabbit polyclonal anti-STAT2 (Cell signaling Technology, Cat#4594S), rabbit polyclonal anti-PY STAT2 (Cell signaling Technology, Cat#4441S), rabbit monoclonal IRF9 (Cell signaling Technology, Cat#28492), and horseradish peroxidase-conjugated secondary antibody (1:5,000).

    Techniques: