polyclonal rabbit anti phospho stat3 s727 cell signaling technology (Cell Signaling Technology Inc)
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Polyclonal Rabbit Anti Phospho Stat3 S727 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 248 article reviews
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Incubation:Article Title: Fostamatinib (R788), a spleen tyrosine kinase inhibitor, sensitizes pancreatic cancer cells to oncolytic vesicular stomatitis virus Article Snippet: .. Membranes were then incubated in TBS-T with 5% BSA or milk with 0.02% sodium azide and a 1:5,000 dilution of rabbit polyclonal anti-VSV antibodies (raised against VSV virions), a 1:1,000 dilution of rabbit anti-phospho-STAT1 (catalog number 9167S, Cell Signaling), a 1:1,000 dilution of rabbit anti-STAT1 (catalog number 14994T, clone D1K9Y, Cell Signaling), a 1:1,000 dilution of rabbit anti-phospho-STAT2 (catalog number PA5-97361clone p-Y690, Invitrogen), a 1:1,000 dilution of Article Title: Distinct ZIKV strain signatures and type I IFN modulation reveal a protective role of brain endothelial interferon signaling in vitro and in vivo Article Snippet: The samples were subjected to a 10% polyacrylamide gel electrophoresis (SDS-PAGE), followed by transfer to a nitrocellulose membrane (Merck). .. The membranes were blocked with 10% TBS containing 5% bovine serum albumin (BSA; Merck) for 1 h, and incubated with the antibodies anti-p-STAT1, anti-STAT1, anti-p-STAT2, Article Title: A 20-amino-acid Cardiovirus protein exhibits cytokine-mimicry activity to regulate viral replication. Article Snippet: The PVDF membrane was pre-soaked in methanol (Nacalai Tesque Inc., 21915-93), and transferred using transfer buffer (25mM Tris-base, 192mM glycine (Nacalai Tesque Inc., 17109-35), 20% methanol). .. After the transfer process, the PVDF membrane was blocked using 5% BSA (Nacalai Tesque Inc., 01863-48) in 1X TBS-T (20mM Tris-HCl pH 8.0, 150mM NaCl, 0.1% Tween 20 (Nacalai Tesque Inc., 35624-15) for 60 min, and incubated with primary antibodies (diluted with 5% BSA in TBS-T) overnight at 4 ◦ C. Dilution of primary antibodies: Anti-GAPDH (Santa Cruz Biotechnology, sc-32233): 1:1000, Anti-caspase-8 (CST, #9746): 1:500, Anti-Cleaved PARP (CST, #9541): 1:1000, Anti-VPg EMCV (IBL, This study): 1:5000, Anti-Mengo 3DPol (Santa Cruz Biotechnology, sc-65633): 1:500, Anti-iNOS (Santa Cruz Biotechnology, sc-7271): 1:500, Anti-STAT1 (CST, #9172): 1:1000, Anti-pSTAT1 (Y701) (CST, #9167): 1:1000, Western Blot:Article Title: Distinct ZIKV strain signatures and type I IFN modulation reveal a protective role of brain endothelial interferon signaling in vitro and in vivo Article Snippet: The samples were subjected to a 10% polyacrylamide gel electrophoresis (SDS-PAGE), followed by transfer to a nitrocellulose membrane (Merck). .. The membranes were blocked with 10% TBS containing 5% bovine serum albumin (BSA; Merck) for 1 h, and incubated with the antibodies anti-p-STAT1, anti-STAT1, anti-p-STAT2, Article Title: Crimean-Congo hemorrhagic fever virus NSm protein inhibits the type I interferon signaling by binding to STAT2. Article Snippet: .. Western blotting was performed as previously described using the following antibodies [10,12,13]: anti-HA (clone 3F10; Roche), anti-His (clone 9F2; Fujifilm Wako), anti-FLAG (catalog no. PM020; MBL), anti-VSIV M (clone 23H12; Kerafast), anti-VSIV G (clone P5D4; Sigma-Aldrich), Article Title: Crimean-Congo hemorrhagic fever virus NSm protein inhibits the type I interferon signaling by binding to STAT2 Article Snippet: .. Western blotting was performed as previously described using the following antibodies [ , , ]: anti-HA (clone 3F10; Roche), anti-His (clone 9F2; Fujifilm Wako), anti-FLAG (catalog no. PM020; MBL), anti-VSIV M (clone 23H12; Kerafast), anti-VSIV G (clone P5D4; Sigma-Aldrich), Membrane:Article Title: A 20-amino-acid Cardiovirus protein exhibits cytokine-mimicry activity to regulate viral replication. Article Snippet: The PVDF membrane was pre-soaked in methanol (Nacalai Tesque Inc., 21915-93), and transferred using transfer buffer (25mM Tris-base, 192mM glycine (Nacalai Tesque Inc., 17109-35), 20% methanol). .. After the transfer process, the PVDF membrane was blocked using 5% BSA (Nacalai Tesque Inc., 01863-48) in 1X TBS-T (20mM Tris-HCl pH 8.0, 150mM NaCl, 0.1% Tween 20 (Nacalai Tesque Inc., 35624-15) for 60 min, and incubated with primary antibodies (diluted with 5% BSA in TBS-T) overnight at 4 ◦ C. Dilution of primary antibodies: Anti-GAPDH (Santa Cruz Biotechnology, sc-32233): 1:1000, Anti-caspase-8 (CST, #9746): 1:500, Anti-Cleaved PARP (CST, #9541): 1:1000, Anti-VPg EMCV (IBL, This study): 1:5000, Anti-Mengo 3DPol (Santa Cruz Biotechnology, sc-65633): 1:500, Anti-iNOS (Santa Cruz Biotechnology, sc-7271): 1:500, Anti-STAT1 (CST, #9172): 1:1000, Anti-pSTAT1 (Y701) (CST, #9167): 1:1000, |

